Mosalaganti Lab

We use advanced microscopy to understand how multi-protein cellular complexes perform their functions within the context of the cell environment — and how these functions contribute to health and disease.

We seek to understand how membrane-enclosed structures within our cells perform their specific jobs to maintain cell health. We are particularly interested in lysosomes, which not only play an important role in cellular 'waste management,' but also  serve as a key decision-making center controlling cellular growth and survival. Loss of these functions can lead to wide range of diseases, including cancer and cardiovascular disorders.

Our lab combines state-of-the-art cryo-electron microscopy/tomography and cryo-confocal microscopy approaches with biochemical and cell biology methods to elucidate how lysosomes perform their functions and engage in clearance of pathological aggregates. We are also interested in structural characterization of lysosomal membrane transporters and channels.

Shyamal Mosalaganti, Ph.D.

Research Assistant Professor, U-M Life Sciences Institute
Assistant Professor of Cell and Developmental Biology, U-M Medical School
Assistant Professor of Biophysics, U-M College of Literature, Science, and the Arts

Publication Highlights

AI-based structure prediction empowers integrative structural analysis of human nuclear pores

Mosalaganti S, Obarska-Kosinska A, Siggel M, Taniguchi R, Turoňová B, Zimmerli CE, Buczak K, Schmidt FH, Margiotta E, Mackmull MT, Hagen WJH, Hummer G, Kosinski J, Beck M. Science (2022)

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

Turoňová B, Sikora M, Schürmann C, Hagen W, Welsch S, Blanc FC, Von Bülow S, Gecht M, Bagola K, Hörner C, Van Zandbergen G, Mosalaganti S, Schwarz A, Covino R, Mühlebach MD, Hummer G, Locker JK, Beck M. Science (2020)

In situ architecture of the algal nuclear pore complex

Mosalaganti S*, Kosinski J*, Albert S*, Schaffer M*, Strenkert D, Salomé PA, Merchant SS, Plitzko JM,  Baumeister W, Engel BD, Martin Beck M. Nature Communications (2018)

Structure of the RZZ complex and molecular basis of its interaction with Spindly

Mosalaganti S*, Keller J*, Altenfeld A*, Winzker M, Rombaut P, Saur M, Petrovic A, Wehenkel A, Wohlgemuth S, Müller F, Maffini S, Bange T, Herzog F, Waldmann H, Raunser S, Musacchio A. J Cell Biol (2017)

Molecular architecture of the inner ring scaffold of the human nuclear pore complex

Kosinski J*, Mosalaganti S*, von Appen A*, Teimer R, DiGuilio AL, Wan W, Bui KH, Hagen WJ, Briggs JA, Glavy JS, Hurt E, Beck M. Science (2016)

Modular assembly of RWD domains on the Mis12 complex underlies outer kinetochore organization.

Petrovic A*, Mosalaganti S*, Keller J*, Mattiuzzo M, Overlack K, Krenn V, De Antoni A, Wohlgemuth S, Cecatiello V, Pasqualato S, Raunser S, Musacchio A. Mol Cell (2014)

*Co-first authors
Structure of the human heparan-α-glucosaminide N-acetyltransferase (HGSNAT)

Navratna V, Kumar A, Rana JK, Mosalaganti S. eLife (reviewed preprint; 2023)

Mosalaganti Lab

Room 3163
Life Sciences Institute
Mary Sue Coleman Hall
210 Washtenaw Avenue
Ann Arbor, MI 48109-2216