Jay Brito Querido Lab

We use single-particle cryo-electron microscopy in combination with biochemical approaches to study how messenger RNA translation is regulated in human health and disease.

Our Research

The process of translating messenger RNA (mRNA) into proteins must be tightly regulated. Our lab is focused particularly on how the process is initiated.

Using cryo-electron microscopy in combination with biochemical and genetic approaches, we study the role of a family of enzymes called DEAD-box helicases in the initiation of mRNA translation in humans, and how this initiation is regulated in health and disease. 

Jay Brito Querido

Publication Highlights

The structure of a human translation initiation complex reveals two independent roles for the helicase eIF4A

Brito Querido J, Sokabe M, Díaz-López I, Gordiyenko Y, Fraser CS, Ramakrishnan V, Nat Struct Mol Biol (2024) 

The molecular basis of translation initiation and its regulation in eukaryotes

Brito Querido J, Díaz-López I, Ramakrishnan V, Nat Rev Mol Cell Biol (2023)

Structure of a human 48S translational initiation complex

Brito Querido J*, Sokabe M*, Kraatz S*, Gordiyenko Y, Shehel M, S. Fraser C, Ramakrishnan V, Science (2020)

Structural differences in translation initiation between pathogenic trypanosomatids and their mammalian hosts

Bochler A*, Brito Querido J*, Prilepskaja T*, Soufari H, Simonetti A, Lucia Del Cistia M, Kuhn L, Rimoldi Ribeiro A, Shivaya Valášek L, Hashem Y, Cell Reports (2020)

The cryo-EM structure of a novel 40S kinetoplastid-specific ribosomal protein

Brito Querido J*, Mancera-Martınez E*, Vicens Q, Bochler A, Chicher J, Simonetti A, Hashem Y, Structure (2017)

Jay Brito Querido
Department of Biological Chemistry, Michigan Medicine
Life Sciences Institute
Mary Sue Coleman Hall
210 Washtenaw Avenue
Ann Arbor, MI 48109-2216