Screening Services

Flow Cytometry-Based High-Throughput Screening

Bio-Rad ZE5 flow cytometer, in collaboration with the BCRF Flow cytometry core, for multi-parameter and high throughput screening. Visit the Flow Core website.

Image-Based High-Throughput Screening

Image-based HTS (using IXM) allows phenotypic analysis of small molecule and siRNA screens. These assays can be performed in multiwell plates with kinetic or endpoint reads. Analysis of images is user-defined, automatic, and multi-parameter.

Compound High-Throughput Screening (HTS)

  • >200,000 small molecules (diversity sets)
  • Kinase activity libraries
  • Bioactive collections (clinically tested)
  • Drug-like compound library
  • >50,000 natural product extracts (novel collection)

RNA Interference High-Throughput Screening

  • Dharmacon siGenome SMARTpool library:
    • Human Genome siRNA set targets 18,200 genes, 68 source plates, 384-well format
    • Druggable targets, Subset targets, 3520 genes, 96- or 384-well format
    • Mouse Genome siRNA set targets 16,873 genes, 63 source plates, 384-well format

Instruments/Technology Platforms

  • Detection formats: 96-, 384-  & 1536-well plates
  • High-Content Screening: Yokogawa CV8000 with BRCF Microscopy
  • Flow Cytometer: Bio-Rad ZE5, high-throughput 96 and 384 well 
  • Two Multimode plate readers: Abs, FI, FP, Lum, FRET, BRET, ALPHA, TR-FRET, HTRF
  • Hamamatsu FDSS 7000 high-throughput kinetic fluorescence and luminescence imaging plate reader

Robotics, Liquid Handling, Compound Plates

  • Biomek automated microliter volume dispensing
  • Echo Acoustic automated nanoliter volume dispensing
  • Multidrop Combi bulk liquid handlers
  • Integra Assist Plus and Viaflow 384 liquid handlers
  • Multi-dispense/multichannel pipettors
  • Custom compound plate formats for secondary screening, pilot assays and non-HTS uses
  • Relational database of assays and compounds
  • Screening, chemoinformatics and in silico support
  • Structure similarity searches and links to public databases for chemical and biological profiling are available and free for CCG users
  • Support for secondary screening experiments, toxicology assessment & ADME
  • Ca++ Flux, Ion Channel, Membrane Potential
  • High-content automated image analysis on multiwell plate formats and slides Kinetic reads over hours or days