The NATx Core lab is designed to provide nucleic acid synthesis at multiple scales and throughputs. Our state-of-the-art equipment, along with our team’s specialized training, ensures the prompt delivery of high-quality oligos and oligo-conjugates to support your research needs.
Synthesis Equipment
Dr. Oligo 48 (Biolytic)
- Synthesis of up to 48 oligonucleotides simultaneously
- 42 reagent positions for diverse chemical modifications
- 200 nmol – 5 µmol synthesis scale
K&A H-8-VLS (Sierra Biosystems)
- Synthesis of 8 oligos
- 16 reagent positions
- 1 µmol – 100 µmol synthesis scale
Purification & QC Equipment
1290 Infinity III Preparative HPLC & 1260 Infinity III Analytical HPLC (Agilent)
- Ultrahigh-performance liquid chromatography (UHPLC)
- Compound isolation using high-salt and extreme-pH conditions
Endotoxin Monitor (Charles River)
- Supports greater confidence in materials intended for sensitive biological assays
Validation and Quantification Equipment
1290 Infinity III LC and TOF-MS (Agilent)
NanoPhotometer N120 Multi-Channel (Implen)
- 12-channel microvolume spectrophotometer
- 20 seconds per full run
- 200 – 900 nm scan range
- Combines fluorescence, DLS and UV-Vis capabilities for nucleic acid and antibody-oligo conjugate quantitation, as well as LNP quantitation and characterization
- 96-well format, 2 uL per sample in microfluidic chamber
Screening Equipment
Quant Studio 3 and 5 PCR (Applied Biosystems)
- One-step RT-qPCR
- 96- and 384-well formats
Biorad QX700 HT (Temporary placement)
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Digital droplet PCR for direct quantification of nucleic acids