The NATx Core lab is designed to provide nucleic acid synthesis at multiple scales and throughputs. Our state-of-the-art equipment, along with our team’s specialized training, ensures the prompt delivery of high-quality oligos and oligo-conjugates to support your research needs.
Synthesis Equipment
Dr. Oligo 48 (Biolytic) – Small-scale, high-throughput oligonucleotide synthesis
- Synthesis of up to 48 oligonucleotides simultaneously
- 42 reagent positions to accommodate diverse chemical modifications
- Synthesis scales ranging from 200 nmol to 5 µmol
K&A H-8-VLS (Sierra Biosystems) – Mid- to large-scale oligonucleotide synthesis
- Synthesis of 8 oligonucleotides simultaneously
- 16 reagent positions to accommodate for diverse chemical modification
- Synthesis scales ranging from 1 µmol to 100 µmol
Purification & QC Equipment
1290 Infinity III Preparative HPLC & 1260 Infinity III Analytical HPLC (Agilent)
- High-resolution purification and analytical characterization of oligonucleotides
- Separation modes include ion-exchange, reverse phase, and size exclusion chromatograph
Endotoxin Monitor (Charles River)
- Supports greater confidence in materials intended for sensitive biological assays
Validation and Quantification Equipment
1290 Infinity III LC and TOF-MS (Agilent)
- High-resolution accurate mass analysis of synthesized oligonucleotides
- BioConfirm software enabling the detection and characterization of synthesis-related impurities and byproducts
- Ion-pair reverse-phase (IP-RP_ LC-MS using HFIP-based mobile phase for high sensitivity oligonucleotide detection)
NanoPhotometer N120 Multi-Channel (Implen)
- 12-channel microvolume spectrophotometer
- 20 seconds per full run
- 200 – 900 nm scan range
- Combines fluorescence, DLS and UV-Vis capabilities for nucleic acid and antibody-oligo conjugate quantitation, as well as LNP quantitation and characterization
- 96-well format, 2 uL per sample in microfluidic chamber
Screening Equipment
Quant Studio 3 and 5 PCR (Applied Biosystems)
- One-step RT-qPCR
- 96- and 384-well formats
Biorad QX700 HT (Temporary placement)
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Digital droplet PCR for direct quantification of nucleic acids