Nuclear Magnetic Resonance Suite

Housed in the third floor of the LSI, the Varian 400 MHz NMR and 600 MHz NMR are modern, high-end active-shielded instruments suitable for biomolecule structural analysis. There is also a Protasis CapNMR Probe, which allows unprecedented reduction in sample volume for NMR analysis, in turn reducing solvent usage and permits detection of small quantities of organic molecules. This system provides rapid structural determination of compounds from HPLC fraction high throughput.


A state-of-the-art Varian 400 NMR spectrometer has pulsed field gradients, auto shim and a 5mm PFG ATB Broadband probe. The state-of-the-art Varian 600 NMR spectrometer has pulsed field gradients, auto shim and a 5mm Broadband probe. They are both "research grade" instruments capable of performing all of the solution NMR techniques commonly reported in the literature. Commonly performed experiments include simple spectra, T1 and T2 relaxation experiments, solvent suppression, selective decoupling and nOe experiments, as well as two-dimensional (2D) COSY, NOESY, HSQC and HMBC. Other experiments required by the user can be implemented with the aid and supervision of the spectroscopist.

Scheduling and Sample Preparation

Training: In most cases the spectroscopist will train investigators in the use of the NMR and provide expertise and troubleshooting of NMR instrumental problems as needed. In a few cases it may be necessary for the spectroscopist to perform the experiment. The decision will be made at the discretion of the spectroscopist.

Instrument Use: Trained/Approved users can reserve time on these machines through the U of M CTools site. Although instrument time is occasionally available on short notice, investigators should not rely on immediate access. Priority will generally be given to users who run their own experiments. The Varian 400 and 600 are “walk on” instruments that can be used by trained users.  

Sample Preparation: NMR sample preparation is the responsibility of the user. It is also the user's responsibility to provide their own NMR tubes, deuterated solvents and pipets. The NMR tubes should be of high grade. Contact the manager for minimal NMR tube specifications and for advice on sample preparation.

Facility Personnel

Wendy Feng, Ph.D., Spectroscopist
wendyfen [at]


Anna Mapp, Faculty Co-Director, LSI NMR Facility
amapp [at]

David Sherman, Faculty Co-Director, LSI NMR Facility
davidhs [at]

Cathy Andrews, LSI Director of Operations
cathyand [at]


Life Sciences Institute
Room 3214
210 Washtenaw Ave.
University of Michigan
Ann Arbor, Michigan 48109-2216
Phone 734-763-1200