Our lab is interested in understanding how large molecular machines are structurally organized and how this organization translates into function within the cell. We use single particle cryo-electron microscopy, as well as a combination of biological and biochemical techniques to pursue this goal.
- cryo-electron microscopy
Melanie Ohi's reserach focuses on dissecting the structural and functional organization of the spliceosome, generating mechanistic models for how pore-forming toxins secreted by pathogenic bacteria transition from soluble to membrane inserted states, and studying the ways proteins can structurally influence membrance organization.
Pyburn TM, Foegeding NJ, González-Rivera C, McDonald NA, Gould KL, Cover TL, Ohi MD. Structural organization of membrane-inserted hexamers formed by Helicobacter pylori VacA toxin. Molecular Microbiology. 2016 Jun 16.
Foegeding NJ, Caston RR, McClain MS, Ohi MD, Cover TL. An Overview of Helicobacter pylori VacA Toxin Biology. Toxins. 2016 Jun 3;6(8). pii: E173.
McDonald NA, Takizawa Y, Feoktistova A, Xu P, Ohi MD, Vander Kooi CW, Gould KL. The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis. Cell reports. 2016 Jan 26;14(3). 534-46.
Frick-Cheng AE, Pyburn TM, Voss BJ, McDonald WH, Ohi MD, Cover TL. Molecular and Structural Analysis of the Helicobacter pylori cag Type IV Secretion System Core Complex. MBio. 7(1).