Inoki publications

Recent Articles

Yao Y, Wang J, Yoshida S, Nada S, Okada M, Inoki K. (2016) Role of Ragulator in the Regulation of Mechanistic Target of Rapamycin Signaling in Podocytes and Glomerular Function. J Am Soc Nephrol. Mar 31. doi: 10.1681/ASN.2015010032 [Epub ahead of print]. PubMed link.  

Yao Y, Inoki K. (2016) The role of mechanistic target of rapamycin in maintenance of glomerular epithelial cells. Curr Opin Nephrol Hypertens. Jan;25(1):28-34. doi: 10.1097/MNH.0000000000000181. PMCID: PMC4911704. PubMed link.

Yoshida S, Pacitto R, Yao Y, Inoki K, Swanson JA. (2015) Growth factor signaling to mTORC1 by amino acid-laden macropinosomes. J Cell Biol. Oct 12;211(1):159-72. doi: 10.1083/jcb.201504097. PMCID: PMC4602043. PubMed link.

Park DW, Jiang S, Liu Y, Siegal GP, Inoki K, Abraham E, Zmijewski JW. (2014) GSK3β-dependent inhibition of AMPK potentiates activation of neutrophils and macrophages and enhances severity of acute lung injury. Am J Physiol Lung Cell Mol Physiol. Nov 15;307(10):L735-45. doi: 10.1152/ajplung.00165.2014. PMCID: PMC4233296. PubMed link.

Zhang D, Tong X, Arthurs B, Guha A, Rui L, Kamath A, Inoki K, Yin L. (2014) Liver clock protein BMAL1 promotes de novo lipogenesis through insulin-mTORC2-AKT signaling. J Biol Chem. Sep 12;289(37):25925-35. doi: 10.1074/jbc.M114.567628. PMCID: PMC4162191. PubMed link.

Hong S, Zhao B, Lombard DB, Fingar DC, Inoki K. (2014) Cross-talk between sirtuin and mammalian target of rapamycin complex 1 (mTORC1) signaling in the regulation of S6 kinase 1 (S6K1) phosphorylation. J Biol Chem. May 9;289(19):13132-41. doi: 10.1074/jbc.M113.520734. PMCID: PMC4036325. PubMed link

Tang H, Inoki K, Lee M, Wright E, Khuong A, Khuong A, Sugiarto S, Garner M, Paik J, DePinho RA, Goldman D, Guan KL, Shrager JB. (2014) mTORC1 promotes denervation-induced muscle atrophy through a mechanism involving the activation of FoxO and E3 ubiquitin ligases. Sci Signal. Feb 25;7(314):ra18. doi: 10.1126/scisignal.2004809. PMID: 24570486. PubMed link.

Inoki K. (2014) mTOR signaling in autophagy regulation in the kidney. Semin Nephrol. Jan;34(1):2-8. doi: 10.1016/j.semnephrol.2013.11.002. PMCID: PMC4911697. PubMed link.

Inoki K. (2013) Proximal tubules forget "self-eating" when they meet Western meals. J Am Soc Nephrol. Nov;24(11):1711-3. doi: 10.1681/ASN.2013070794. PMCID: PMC3810096. PubMed link.

Suzuki T, Bridges D, Nakada D, Skiniotis G, Morrison SJ, Lin J, Saltiel AR, Inoki K. (2013) Inhibition of AMPK catabolic action by GSK3. Mol Cell. May 9;50(3):407-19. doi: 10.1016/j.molcel.2013.03.022. PubMed link.

Narita M, Inoki K. (2012) Rags connect mTOR and autophagy. Small GTPases. Apr-Jun;3(2):111-4. doi: 10.4161/sgtp.19422. PMID: 22790199. PubMed link.

Henry FE, McCartney AJ, Neely R, Perez AS, Carruthers CJ, Stuenkel EL, Inoki K, Sutton MA. (2012) Retrograde changes in presynaptic function driven by dendritic mTORC1. J Neurosci. 32(48):17128-42. doi: 10.1523/JNEUROSCI.2149-12.2012. PubMed link

Fukuda A, Chowdhury MA, Venkatareddy MP, Wang SQ, Nishizono R, Suzuki T, Wickman LT, Wiggins JE, Muchayi T, Fingar D, Shedden KA, Inoki K, Wiggins RC. (2012) Growth-dependent podocyte failure causes glomerulosclerosis. J Am Soc Nephrol. 23(8):1351-63. doi: 10.1681/ASN.2012030271. PubMed link

Bartholomew CR, Suzuki T, Du Z, Backues SK, Jin M, Lynch-Day MA, Umekawa M, Kamath A, Zhao M, Xie Z, Inoki K, Klionsky DJ. (2012) Ume6 transcription factor is part of a signaling cascade that regulates autophagy. Proc Natl Acad Sci USA. 109(28):11206-10. doi: 10.1073/pnas.1200313109. PubMed link

Bridges D, Ma JT, Park S, Inoki K, Weisman LS, Saltiel AR. (2012) Phosphatidylinositol 3,5-bisphosphate plays a role in the activation and subcellular localization of mechanistic target of rapamycin 1. Mol Biol Cell. 23(15):2955-62. doi: 10.1091/mbc.E11-12-1034. PubMed link

Huber TB, Edelstein CL, Hartleben B, Inoki K, Dong Z, Koya D, Kume S, Lieberthal W, Pallet N, Quiroga A, Ravichandran K, Susztak K, Yoshida S, Dong Z. (2012) Emerging role of autophagy in kidney function, diseases and aging. Autophagy. 8(7):1009-31. doi: 10.4161/auto.19821. PubMed link

Bridges D, Fisher K, Zolov SN, Xiong T, Inoki K, Weisman LS, Saltiel AR. (2012) Rab5 proteins regulate activation and localization of target of rapamycin complex 1. J Biol Chem. 287(25):20913-21. doi: 10.1074/jbc.M111.334060.  PubMed link

Yoshida S, Hong S, Suzuki T, Nada S, Mannan AM, Wang J, Okada M, Guan KL, Inoki K. (2011) Redox Regulates Mammalian Target of Rapamycin Complex 1 (mTORC1) Activity by Modulating the TSC1/TSC2-Rheb GTPase Pathway. J Biol Chem. 286(37):32651-60. PubMed link

Inoki K, Mori H, Wang J, Suzuki T, Hong S, Yoshida S, Blattner SM, Ikenoue T, Rüegg MA, Hall MN, Kwiatkowski DJ, Rastaldi MP, Huber TB, Kretzler M, Holzman LB, Wiggins RC, Guan KL. (2011) mTORC1 activation in podocytes is a critical step in the development of diabetic nephropathy in mice. J Clin Invest. pii: 44771. doi: 10.1172/JCI44771. PubMed link

Gödel M, Hartleben B, Herbach N, Liu S, Zschiedrich S, Lu S, Debreczeni-Mór A, Lindenmeyer MT, Rastaldi MP, Hartleben G, Wiech T, Fornoni A, Nelson RG, Kretzler M, Wanke R, Pavenstädt H, Kerjaschki D, Cohen CD, Hall MN, Rüegg MA, Inoki K, Walz G, Huber TB. (2011) Role of mTOR in podocyte function and diabetic nephropathy in humans and mice. J Clin Invest. pii: 44774. doi: 10.1172/JCI44774. PubMed link

Narita M, Young AR, Arakawa S, Samarajiwa SA, Nakashima T, Yoshida S, Hong S, Berry LS, Reichelt S, Ferreira M, Tavaré S, Inoki K, Shimizu S, Narita M. (2011) Spatial coupling of mTOR and autophagy augments secretory phenotypes. Science. 332(6032):966-70. PubMed link

Li L, Kim E, Yuan H, Inoki K, Goraksha-Hicks P, Schiesher RL, Neufeld TP, Guan KL. (2010) Regulation of mTORC1 by the Rab and Arf GTPases. J Biol Chem. 285(26):19705-9. PubMed link

Alexander A, Cai SL, Kim J, Nanez A, Sahin M, MacLean KH, Inoki K, Guan KL, Shen J, Person MD, Kusewitt D, Mills GB, Kastan MB, Walker CL. (2010) ATM signals to TSC2 in the cytoplasm to regulate mTORC1 in response to ROS. Proc Natl Acad Sci USA. 107(9):4153-8. PubMed link

 Izumi K, Inoki K, Fujimori Y, Marcelo CL, Feinberg SE. (2009). Pharmacological Retention of Oral Mucosa Progenitor/Stem Cells. J Dent Res. PubMed link 

Diallo-Krou E, Yu J, Colby LA, Inoki K, Wilkinson JE, Thomas DG, Giordano TJ, Koenig RJ. (2009). Paired box gene 8-peroxisome proliferator-activated receptor-gamma fusion protein and loss of phosphatase and tensin homolog synergistically cause thyroid hyperplasia in transgenic mice. Endocrinology. 150(11):5181-90. PubMed link

Mori H, Inoki K, Opland D, Muenzberg H, Villanueva EC, Faouzi M, Ikenoue T, Kwiatkowski D, Macdougald OA, Myers Jr MG, Guan KL. (2009). Critical roles for the TSC-mTOR pathway in {beta}-cell function. Am J Physiol Endocrinol Metab. PubMed link

Zhou X, Ikenoue T, Chen X, Li L, Inoki K, Guan KL. (2009). Rheb controls misfolded protein metabolism by inhibiting aggresome formation and autophagy. Proc Natl Acad Sci USA. 106(22):8923-8. PubMed link

Mori H, Inoki K, Masutani K, Wakabayashi Y, Komai K, Nakagawa R, Guan KL, Yoshimura A. (2009). The mTOR pathway is highly activated in diabetic nephropathy and rapamycin has a strong therapeutic potential. Biochem Biophys Res Commun. 384(4):471-5. PubMed link

Mori, H. Inoki, K., Munzberg, H., Opland, D., Faouzi, M., Villanueva, E.C., Ikenoue, T., Kwiatkowski, D., MacDougald, O.A., Myers, M.G. Jr., and Guan, K.L. (2009). Critcal Role for Hypothalamic mTOR Activity in Energy Balance. Cell Metabolism. 9(4):362-74. PubMed link

Ikenoue, T., Inoki, K., Zhao, B., Guan, K.L. (2008). PTEN acetylation modulates its interaction with PDZ domain. Cancer Res. 68(17), 6908-12. PubMed link

Buller, C.L., Loberg, R.D., Fan, M.H., Zhu, Q., Park, J.L., Vesely, E., Inoki, K., Guan, K.L., Brosius, F.C. 3rd. (2008) A GSK-3/TSC2/mTOR pathway regulates glucose uptake and GLUT1 glucose transporter expression. Am J Physiol Cell Physiol. 295(3), C836-43. PubMed link

Ikenoue, T., Inoki, K., Yang, Q., Zhou, X., Guan, K.L. (2008) Essential function of TORC2 in PKC and Akt turn motif phosphorylation, maturation and signalling. EMBO J. 27(14), 1919-31. PubMed link

Lee, C.H., Inoki, K., Karbowniczek, M., Petroulakis, E., Sonenberg, N., Henske, E.P., and Guan, K.L. (2007). Constitutive mTOR activation in TSC mutants sensitizes cells to energy starvation and genomic damage via p53. EMBO J 26(23), 4812-23. PubMed link

Inoki, K., Ouyang, H., Zhu, T., Lindvall, C., Wang, Y., Zhang, X., Yang, Q., Bennett, C., Harada, Y., Stankunas, K., Wang, CY., He, X., MacDougald, OA., You, M., Williams, BO., and Guan, K. L. (2006). TSC2 integrates Wnt and energy signals via a coordinated phosphorylation by AMPK and GSK3 to regulate cell growth. Cell 126, 955-968. PubMed link

Yang, Q., Inoki, K., Kim, E., and Guan, K. L. (2006). TSC1/TSC2 and Rheb have different effects on TORC1 and TORC2 activity. Proc Natl Acad Sci USA 103, 6811- 6816. PubMed link

Chong-Kopera, H., Inoki, K., Li, Y., Zhu, T., Garcia-Gonzalo, F. R., Rosa, J. L., and Guan, K. L. (2006). TSC1 stabilizes TSC2 by inhibiting the interaction between TSC2 and the HERC1 ubiquitin ligase. J Biol Chem 281, 8313-8316. PubMed link

Yang, Q., Inoki, K., Ikenoue, T., and Guan, K. L. (2006). Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity. Genes Dev 20, 2820-2832. PubMed link

Guo, B., Inoki, K., Isono, M., Mori, H., Kanasaki, K., Sugimoto, T., Akiba, S., Sato, T., Yang, B., Kikkawa, R., et al. (2005). MAPK/AP-1-dependent regulation of PAI-1 gene expression by TGF-beta in rat mesangial cells. Kidney Int 68, 972-984. PubMed link

Corradetti, M. N., Inoki, K., and Guan, K. L. (2005). The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway. J Biol Chem 280, 9769-9772. PubMed link

Li, Y., Inoki, K., and Guan, K. L. (2004). Biochemical and functional characterizations of small GTPase Rheb and TSC2 GAP activity. Mol Cell Biol 24, 7965-7975. PubMed link

Corradetti, M. N., Inoki, K*., Bardeesy, N., DePinho, R. A., and Guan, K. L*. (2004). Regulation of the TSC pathway by LKB1: evidence of a molecular link between tuberous sclerosis complex and Peutz-Jeghers syndrome. Genes Dev 18, 1533-1538. (*co-corresponding) PubMed link

Articles (1999-2003)

Inoki, K., Zhu, T., and Guan, K. L. (2003). TSC2 mediates cellular energy response to control cell growth and survival. Cell 115, 577-590. PubMed link

Inoki, K., Li, Y., Xu, T., and Guan, K. L. (2003). Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Dev 17, 1829-1834. PubMed link

Li, Y., Inoki, K., Vacratsis, P., and Guan, K. L. (2003). The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3. J Biol Chem 278, 13663-13671. PubMed link

Li, Y., Inoki, K., Yeung, R., and Guan, K. L. (2002). Regulation of TSC2 by 14-3-3 binding. J Biol Chem 277, 44593-44596. PubMed link

Inoki, K., Li, Y., Zhu, T., Wu, J., and Guan, K. L. (2002). TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling. Nat Cell Biol 4, 648-657. PubMed link

Nishio, T., Haneda, M., Koya, D., Inoki, K., Maeda, S., and Kikkawa, R. (2002). Cyclic AMP inhibits stretch-induced overexpression of fibronectin in glomerular mesangial cells. Eur J Pharmacol 437, 113-122. PubMed link

Sawano, H., Haneda, M., Sugimoto, T., Inoki, K., Koya, D., and Kikkawa, R. (2002). 15-Deoxy-Delta12,14-prostaglandin J2 inhibits IL-1beta-induced cyclooxygenase-2 expression in mesangial cells. Kidney Int 61, 1957-1967. PubMed link

Sugimoto, T., Haneda, M., Sawano, H., Isshiki, K., Maeda, S., Koya, D., Inoki, K., Yasuda, H., Kashiwagi, A., and Kikkawa, R. (2001). Endothelin-1 induces cyclooxygenase-2 expression via nuclear factor of activated T-cell transcription factor in glomerular mesangial cells. J Am Soc Nephrol 12, 1359-1368. PubMed link

Inoki, K., Haneda, M., Ishida, T., Mori, H., Maeda, S., Koya, D., Sugimoto, T., and Kikkawa, R. (2000). Role of mitogen-activated protein kinases as downstream effectors of transforming growth factor-beta in mesangial cells. Kidney Int Suppl 77, S76-80. PubMed link

Inoki, K., Haneda, M., Maeda, S., Koya, D., and Kikkawa, R. (1999). TGF-beta 1 stimulates glucose uptake by enhancing GLUT1 expression in mesangial cells. Kidney Int 55, 1704-1712. PubMed link

Kohno, M., Yasunari, K., Minami, M., Kano, H., Maeda, K., Mandal, A. K., Inoki, K., Haneda, M., and Yoshikawa, J. (1999). Regulation of rat mesangial cell migration by platelet-derived growth factor, angiotensin II, and adrenomedullin. J Am Soc Nephrol 10, 2495-2502. PubMed link

Review Articles

Narita M, Inoki K. (2012) Rags connect mTOR and autophagy. Small GTPases. 3(2):111-4. doi: 10.4161/sgtp.19422. PubMed link 

Fingar DC, Inoki K. (2012) Deconvolution of mTORC2 "in Silico". Sci Signal. 5(217):pe12. doi: 10.1126/scisignal.2003026.  PubMed link

Inoki K, Huber TB. (2012) Mammalian target of rapamycin signaling in the podocyte. Curr Opin Nephrol Hypertens. 21(3):251-7. doi: 10.1097/MNH.0b013e3283520f38. PubMed link

Hong S, Mannan AM, Inoki K. (2012) Evaluation of the nutrient-sensing mTOR pathway. Methods Mol Biol. 821:29-44. doi: 10.1007/978-1-61779-430-8_3. PubMed link

Inoki K, Kim J, Guan KL. (2012) AMPK and mTOR in cellular energy homeostasis and drug targets. Annu Rev Pharmacol Toxicol. 52:381-400. doi: 10.1146/annurev-pharmtox-010611-134537. PubMed link

Suzuki T, Inoki K. (2011) Spatial regulation of the mTORC1 system in amino acids sensing pathway. Acta Biochim Biophys Sin (Shanghai). 43(9):671-9. doi: 10.1093/abbs/gmr066. PubMed link

Inoki K., Guan KL., (2009). Tuberous sclerosis complex, implication from a rare genetic disease to common cancer treatment. Hum Mol Genet. 18(R1), R94-100. PubMed link

Ikenoue T, Hong S, Inoki, K., (2009). Monitoring mammalian target of rapamycin (mTOR) activity. Methods Enzymol. 452, 165-80. PubMed link

Inoki K., (2008). Role of TSC-mTOR pathway in diabetic nephropathy. Diabetes Res Clin Pract. 82 Suppl 1, S59-62. PubMed link

Lee, C. H., Inoki, K., and Guan, K. L. (2007). mTOR pathway as a target in tissue hypertrophy. Annu Rev Pharmacol Toxicol 47, 443-467. PubMed link

Inoki, K., and Guan, K. L. (2006). Complexity of the TOR signaling network. Inoki, K., and Guan, K. L. (2006). Complexity of the TOR signaling network. Trends Cell Biol 16, 206-212. PubMed link

Inoki, K., Ouyang, H., Li, Y., and Guan, K. L. (2005). Signaling by target of rapamycin proteins in cell growth control. Microbiol Mol Biol Rev 69, 79-100. PubMed link

Li, Y., Inoki, K., Vikis, H., and Guan, K. L. (2005). Measurements of TSC2 GAP Activity Toward Rheb. Methods Enzymol 407, 46-54. PubMed link

Inoki, K., Corradetti, M. N., and Guan, K. L. (2005). Dysregulation of the TSC-mTOR pathway in human disease. Nat Genet 37, 19-24. PubMed link

Li, Y., Corradetti, M. N., Inoki, K., and Guan, K. L. (2004). TSC2: filling the GAP in the mTOR signaling pathway. Trends Biochem Sci 29, 32-38. PubMed link