The HTP lab has created several series of vectors for use in bacterial expression. These vectors are primarily designed to be compatible with Ligation-Independent Cloning (LIC), which makes them useful for high-throughput parallel cloning. Each series of vectors makes use of replication origins from different incompatibility groups in E. coli, which allows them to be used in combination with one another. Each series has a set of fusion tags and promoter combinations to provide for maximum flexibility in expression trial design. Additionally, the CDF and RSF series of vectors were constructed to be relatively small so they can be used to clone very large genes or multiple-gene cassettes.