Publications

Recent Articles

Singh P, Wang B, Maeda T, Palczewski K, Tesmer JJG (2008). Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activation. JBC, Epub. Pubmed Link

Lutz S., Shankaranarayanan A., Coco C., Ridilla M., Nance M.R., Vettel C., Baltus D., Evelyn C.R., Neubig R.R., Wieland T., Tesmer J.J.G. (2007). Structure of Gαq-p63RhoGEF-RhoA complex reveals a pathway for the activation of RhoA by GPCRs. Science, 318, 1923-7.
Abstract | Full Text

Williams S.L., Lutz S., Charlie N.K., Vettel C., Ailion M., Coco C., Tesmer J.J.G., Jorgensen E.M., Wieland T., Miller K.G. (2007). Trio's Rho-specific GEF domain is the missing Gαq effector in C. elegans. Genes Dev., 21, 2731-46.
Pubmed Link

Chen X., Boughton A.P., Tesmer J.J.G., Chen Z. (2007). In situ investigation of heterptrimeric G proteins βγ subunit binding and orientation on membrane bilayers. J Am chem Soc., 129, 12658-9.
Pubmed Link

Gu S., He J., Ho W.T., Ramineni S., Thal D.M., Natesh R., Tesmer J.J.G., Hepler J.R., Heximer S.P. (2007). Unique hydrophobic extension of the RGS2 amphipathic helix domain imparts increased plasma membrane binding and function relative to other RGS R4/B subfamily members. J Biol Chem, 282, 33064-33075.
Pubmed Link

Li, Q., Nance, M.R., Kulikauskas, R., Nyberg, K., Fehon, R., Karplus, P.A., Bretscher, A., & Tesmer, J.J.G. (2007). Self-masking in an Intact ERM-merlin Protein: An Active Role for the Central α-Helical Domain. Journal of Molecular Biology, 365, 1446-1459.
Pubmed Link

Lodowski, D. T., Tesmer, V. M., Benovic, J. L., & Tesmer, J. J. G. (2006). The structure of G protein-coupled receptor kinase (GRK)-6 defines a second lineage of GRKs. Journal of Biological Chemistry, 281, 16785-16793.
PubMed Link

Kreutz, B., Yau, D. M., Nance, M., Tanabe, S., Tesmer, J. J. G., & Kozasa, T. (2006). A new approach to producing functional Gα subunits yields the activated and deactivated structures of Gα 12/13 proteins. Biochemistry, 45, 167-174.
PubMed Link

Lodowski, D. T., Barnhill, J. F., Pyskadlo, R. M., Ghirlando, R., Sterne-Marr, R., & Tesmer, J. J. G. (2005). The role of Gβγ and domain interfaces in the activation of G protein-coupled receptor kinase 2. Biochemistry, 44, 6958-6970.
PubMed Link

Tesmer, V. M., Kawano, T., Shankaranarayanan, A., Kozasa, T., & Tesmer, J. J. G. (2005). Snapshot of activated G proteins at the membrane: The Gα(q)-GRK2-Gβγ complex. Science, 310, 1686-1690.
Abstract | Full Text

- back to top -

Articles (1990-2004)

Kristelly, R., Gao, G., & Tesmer, J. J. G. (2004). Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor. Journal of Biological Chemistry, 279, 47352-47362.
PubMed Link

Day, P. W., Tesmer, J. J. G., Sterne-Marr, R., Freeman, L. C., Benovic, J. L., & Wedegaertner, P. B. (2004). Characterization of the GRK2 binding site of Gα(q). Journal of Biological Chemistry, 279, 53643-53652.
PubMed Link

Lodowski, D. T., Barnhill, J. F., Pitcher, J. A., Capel, W. D., Lefkowitz, R. J., & Tesmer, J. J. G. (2003). Purification, crystallization and preliminary X-ray diffraction studies of a complex between G protein-coupled receptor kinase 2 and Gβ(1)γ(2). Acta Crystallographica Section D – Biological Crystallography, 59, 936-939.

Kristelly, R., Earnest, B. T., Krishnamoorthy, L., & Tesmer, J. J. G. (2003). Preliminary structure analysis of the DH/PH domains of leukemia-associated RhoGEF. Acta Crystallographica Section D - Biological Crystallography, 59, 1859-1862.
PubMed Link

Sterne-Marr, R., Tesmer, J. J. G., Day, P. W., Stracquatanio, R. P., Cilente, J. A. E., O'Connor, K. E., Pronin, A. N., Benovic, J. L., & Wedegaertner, P. B. (2003). G protein-coupled receptor kinase 2/Gα(q/11) interaction - A novel surface on a regulator of G protein signaling homology domain for binding Gα subunits. Journal of Biological Chemistry, 278, 6050-6058.

Lodowski, D. T., Pitcher, J. A., Capel, W. D., Lefkowitz, R. J., & Tesmer, J. J. G. (2003). Keeping G proteins at bay: A complex between G protein-coupled receptor kinase 2 and Gβγ. Science, 300,1256-1262.
Abstract | Full Text

Tesmer, J. J. G., Dessauer, C. W., Sunahara, R. K., Murray, L. D., Johnson, R. A., Gilman, A. G., & Sprang, S. R. (2000). Molecular basis for P-site inhibition of adenylyl cyclase. Biochemistry, 39, 14464-14471.
PubMed Link

Pitcher, J. A., Tesmer, J. J. G., Freeman, J. L. R., Capel, W. D., Stone, W. C.,
& Lefkowitz, R. J. (1999). Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. Journal of Biological Chemistry, 274, 34531-34534.
PubMed Link

Tesmer, J. J. G., Sunahara, R. K., Johnson, R. A., Gosselin, G., Gilman, A. G., & Sprang, S. R. (1999). Two-metal-ion catalysis in adenylyl cyclase. Science, 285, 756-760.
PubMed Link

Tesmer, J. J. G., & Sprang, S. R. (1998). The structure, catalytic mechanism and regulation of adenylyl cyclase. Current Opinion in Structural Biology, 8, 713-719.
PubMed Link

Sunahara, R. K., Beuve, A., Tesmer, J. J. G., Sprang, S. R., Garbers, D. L.,
& Gilman, A. G. (1998). Exchange of substrate and inhibitor specificities between adenylyl and guanylyl cyclases. Journal of Biological Chemistry, 273, 16332-16338.
PubMed Link

Dessauer, C. W., Tesmer, J. J. G., Sprang, S. R., & Gilman, A. G. (1998). Identification of a G(iα) binding site on type V adenylyl cyclase. Journal of Biological Chemistry, 273, 25831-25839.
PubMed Link

Tesmer, J. J. G., Berman, D. M., Gilman, A. G., & Sprang, S. R. (1997). Structure of RGS4 bound to AlF4--activated G(iα 1): Stabilization of the transition state for GTP hydrolysis. Cell, 89, 251-261.
PubMed Link

Tesmer, J. J. G., Sunahara, R. K., Gilman, A. G., & Sprang, S. R. (1997). Crystal structure of the catalytic domains of adenylyl cyclase in a complex with G(sα).GTPγ S. Science, 278, 1907-1916.
PubMed Link

Sunahara, R. K., Tesmer, J. J. G., Gilman, A. G., & Sprang, S. R. (1997). Crystal structure of the adenylyl cyclase activator G(Sα). Science, 278, 1943-1947.
PubMed Link

Tesmer, J. G., Klem, T. J., Deras, M. L., Davisson, V. J., & Smith, J. L.
(1996). The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for two enzyme families. Nature Structural Biology, 3, 74-86.
PubMed Link

Tesmer, J. J. G., Stemmler, T. L., Pennerhahn, J. E., Davisson, V. J., & Smith, J. L. (1994). Preliminary X-ray analysis of Escherichia coli GMP synthetase - determination of anomalous scattering factors for a cysteinyl mercury derivative. Proteins - Structure, Function and Genetics, 18, 394-403.
PubMed Link

Vermersch, P. S., Lemon, D. D., Tesmer, J. J. G., & Quiocho, F. A. (1991). Sugar-binding and crystallographic studies of an arabinose-binding protein mutant (MET108LEU) that exhibits enhanced affinity and altered specificity. Biochemistry, 30, 6861-6866.
PubMed Link

Vermersch, P. S., Tesmer, J. J. G., Lemon, D. D., & Quiocho, F. A. (1990). A pro to gly mutation in the hinge of the arabinose-binding protein enhances binding and alters specificity - sugar-binding and crystallographic studies. Journal of Biological Chemistry, 265, 16592-16603.
PubMed Link

- back to top -

Review Articles

Sterne-Marr, R., Dhami, G. K., Tesmer, J. J. G., & Ferguson, S. S. G. (2004). Characterization of GRK2 RH domain-dependent regulation of GPCR coupling to heterotrimeric G proteins. Methods in Enzymology, 390, 310-336.

Tesmer, J. J. G., Sunahara, R. K., Fancy, D. A., Gilman, A. G., & Sprang, S. R. (2002). Crystallization of complex between soluble domains of adenylyl cyclase and activated G(s)α. Methods in Enzymology, 345, 198-206.

Dessauer, C. W., Tesmer, J. J. G., Sprang, S. R., & Gilman, A. G. (1999). The interactions of adenylate cyclases with P-site inhibitors. Trends in Pharmacological Sciences, 20, 205-210.

-back to top -

Editorial Material

Tesmer, J. J. G. (2006). Hitting the hot spots of cell signaling cascades. Science, 312, 377-378.

Abstract | Full Text

Tesmer, J. J. G. (2005). A seminal study of soluble adenylyl cyclase. Nature Structural & Molecular Biology, 12, 7-8.

- back to top-

Notes

Vermersch, P. S., Tesmer, J. J. G., & Quiocho, F. A. (1992). Protein ligand energetics assessed using deoxy and fluorodeoxy sugars in equilibrium binding and high-resolution crystallographic studies. Journal of Molecular Biology, 226, 923-929.

Meeting Abstracts

Chen, J., Tesmer, J. J. G., Sprang, S. R., Gilman, A. G., & Dessauer, C. W. (2000). Mechanism of G(iα)-mediated inhibition of type V adenylyl cyclase. Biophysical Journal, 78, 285A.

Tesmer, J. J. G., Sunahara, R. K., Gilman, A. G., & Sprang, S. R. (1998). Structural insights into G protein regulation. FASEB Journal, 12 (Suppl. S), A1325.

Gilman, A. G., Sunahara, R., Dessauer, C. W., Duncan, J. A., Kozasa, T., Krumins, A.
M., Tesmer, J. J. G., & Sprang, S. R. (1998). G proteins, RGS proteins, and adenylyl cyclases. Naunyn-Schmiedebergs Archives of Pharmacology, 358 (Suppl. 1), R6.

Sprang, S. R., Coleman, D. E., Lee, E., Berghuis, A. M., Wall, M. A., Mixon, M. B., Tesmer, J. J. G., Berman, D. M., Posner, B. A., & Gilman, A. G. (1997). Structural insights into G-protein signaling. FASEB Journal, 11 (Suppl. S), A1296.

- back to top -