Equipment
Click any of the images below to see a slideshow of equipment photos. Photos are courtesy Scott R. Davis.
XRay Lab | Crystallization Lab | High-Throughput Protein Lab
XRay Lab
LSI 3358
Rigaku RU-300
- Copper rotating anode
- Raxis IV dual imaging plate detector from Molecular Structure Corporation with accompanying SGI O2 computer for data collection and processing
- Smart 2000 CCD from Bruker with accompanying PC computer for data collection and processing
- X-Stream cryo-cooling system
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Crystallization Lab
Honeybee 961
The Honeybee 961 crystallization robot performs miniature protein-crystallization experiments using the sitting-drop technique. The system dispenses volumes as small 100 nanoliters using a 'synQUAD' nanoliter dispenser, which is integrated with a 96-channel, high-speed dispenser for precipitant solutions. A 96-well crystallization plate can be set up in less than 2 minutes. The user-friendly software allows easy set-up of multiple sitting-drop experiments with 'click-and-play' functionality. After training, researchers are able to use the equipment with little or no supervision from the HTP staff.
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High-Throughput Protein Lab
Located in LSI 3238
Biomek FX
The Biomek FX liquid-handling robot is the workhorse of the High-Throughput Protein Lab. It allows rapid and precise execution of experimental steps in high-throughput cloning and expression optimization. The dual-arm Biomek has a 96-channel pipetting head for fast transfer of volumes up to 250 uL. A gripper for moving labware is integrated with the 96-channel head. The second arm of the Biomek has a pipettor with 8 individually controlled channels for transfer of volumes up to 1 mL.
Caliper Labchip 90
The Caliper Labchip 90, pictured here, is a high-throughput replacement for PAGE analysis of protein and DNA samples. The instrument is based on a microfluidic chip. Samples are introduced in a 96- or 384- well plate, and separated proteins or DNAs are detected by laser-induced fluorescence. The separation data can be displayed either as an electropherogram showing analyte peaks or formatted as a virtual gel image. The unit allows for easy switching between DNA and protein sample analysis through selection of several pre-configured assays. The sensitivity of this system is comparable to coomassie-stained gels but the labor and time involved in sample preparation and processing is considerably less.
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For information on HTS equipment, visit the Equipment page on the CCG site.
