Protein Purification
The CSB provides a variety of purification services that can be tailored to your research needs. These services can be provided in the form of personnel training or full contract services.
The Center has:
- Mulitple variable temperature shakers for both large- and small-scale fermentation
- French press – for breaking E.coli cells
- Sonicator with nano and macro tips for breaking E.coli cells
- Microfluidizer - for breaking E.coli cells
- Two Acta FPLCs equipped with a variety of ion exchange, affinity and both preparative and analytical sized gel filtration columns capable of purifying complexes up to 200,000 Da
Access is determined by Purification Protocol and Scale
For small scale (<1 mg), one-step affinity purification, please contact
Clay Brown of the HTP lab.
For large scale (1 – 100 mg), single-step through multi-step purification using a known protocol, contact
Jennifer Meagher.
