Return to High Throughput Protein Lab
A master plate of positive clones will be produced. The clones will be used to transform an expression strain. Overnight cultures of 1 ml will be used to produce glycerol stocks as well as inoculate 0.5 ml cultures for expression trials. After the expression incubation the cells are lysed by chemical additive and target protein is partially purified by metal affinity chromatography in 96-well plate format on the Biomek. Elutions are analyzed for the
protein of interest using the Labchip 90. A spreadsheet containing the project information with the protein concentrations determined by the Labchip 90 will be given to the requesting lab. The glycerol stocks of the clones in the expression strain will be handed off along with all the protocols necessary for reproducing these results at larger scale. The master plate of positive clones will be made available if requested.
Trouble-shooting expression problems
Review data and protocols, do limited bioinformatic analysis, provide HTP protocols, suggest possible experiments, help develop strategy, in some cases run limited trials and analysis.
