Macromolecular Crystallization & Crystallography
The CSB provides a full range of crystallographic services ranging from crystallization through structure analysis and homology modeling. These services can be provided in the form of personnel training or full contract services. To start or discuss the feasibility of a crystallography project, please contact Jeanne Stuckey
to set up a project consultation.
The CSB provides two types of crystallization services. Robotic screening is performed on a new protein or complex for which crystallization conditions are unknown. The robotic screening uses a 96-well format to screen up to three different samples simultaneously (one recharge rate). The samples can be different proteins, different sample concentrations, different protein-ligand complexes, or a single protein and a buffer alone control and/or a ligand+buffer control sample. We recommend running 4 unique screens (96 conditions each) to ensure broad sampling of crystallization space.
The second type of crystallization service is traditional grid screening using the hanging- or sitting-drop method in a 24-well format. This type of screening is used to screen around hits found in the robotic screen and for screening around published conditions for proteins with known crystallization protocols. The number of samples that can be screened simultaneously is dependent on the method used. For protein-drug complexes, we use the sitting drop method in which up to 4 drug complexes can be screened simultaneously.
Crystallization experiments can be monitored either on-site using a microscope or remotely via the Desktop Imager.
Two levels of service for grid screening crystallization are available: 1.) CSB provides labor and supplies, and 2.) CSB provides supplies and the user provides the labor under the advisement of the CSB.