M-Screen Demo
Equipment
High Throughput Screening Equipment
In addition to providing small molecule libraries, the screening facility has the following equipment and resources available:
Biomek FX (Beckman): A programmable robot utilizing both disposable tips and HDR (pin tool) to rapidly transfer compounds from library stock plates to assay plates. 96 and 384-well plate capability.
Hypercyt/Accuri : High Throughput Flow Cytometer combination system that does multiplex flow cytometry on 96-well plates (~ 3 min/plate)
Plate reader: PHERAstar (BMG Labs) with luminescence, fluorescence, fluorescence polarization, time-resolved fluorescence and absorbance detection methods with 96 and 384-well plate capability. Sample sizes from 5-200ul.
FlexStation3 (MD) plate reader that is capable of reading 5 assay formats for kinetic, endpoint, spectrum, well-scanning and “Flex.”.
Plate Handler: Twister (Caliper Life Sciences) microplate handler allows for automated integration of liquid dispensers, plate washers, and other HTS assay components during screening creating a stand alone module.
Bio-Tek ELx405 plate washer/aspirator with 96 to 384-well capability.
Thermo Labsystems multidrop plate dispensers with 96 and 384-well capability.
Tissue culture facilities. Multichannel pipettors.
Pherastar Modules:
- Luminescence (1 channel)
- Fluorescein/FITC (485/520)
- FP-Fluorescein/FITC (485/520A/520B)
- FP-TAMRA (540/590A/590B)
- AMC (350/450)
- AMC2 (370/480)
- TR-FRET (337/520/490)
- FP-Texas Red (575/620A/620B)
- Absorbance (450)
Other filter modules available—request a list from mjlarsen@umich.edu.
For information on HTP equipment, visit the Equipment page on the CSB site.
