To request training on instruments, contact Martha Larsen. In addition to providing small molecule libraries, the screening facility has the following equipment and resources available:
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Sciclone ALH3000 with a V&P pintool, delivery of 50-80 nl volumes; can be used with 1536-well plates; uses Twister plate hotel to deliver source plates. |
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Envision Multimode Plate Reader (Perkin Elmer), two detectors enabling simultaneous dual wavelength reading; enhanced luminescence; Alpha capabilities (AlphaScreen and AlphaLisa); Stackers for automatic plate reading; FP, FI, TR-FRET, Abs, BRET, HTRF; cross talk, calibration and normalization features; Kinetic reads. |
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Mosquito X1 (TTP Labtech), a hit-picking liquid handler with 96- and 384-well plate formats, 25-1200 nL positive displacement tips for confirmation, serial dilutions and reagent/sample transfers. |
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MDS Analytical Technologies-ImageXpress Micro Cellular Imaging and Analysis System for automated acquisition and analysis of images for high throughput cell-based screening. Includes Acuity Xpress Informatics and MDCStore database. Several plate options (6-384-well) and slide mounts are available for high content analysis and screening. |
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Biomek FX dual head (Beckman): A programmable robot, utilizing both disposable tips and HDR (pin tool) to rapidly transfer compounds from library stock plates to assay plates. 96 and 384-well plate capability. |
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Biomek FX (Beckman) 384-well nanoliter HDR (pin tool) |
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Hypercyt/Accuri : High Throughput Flow Cytometer combination system that does multiplex flow cytometry on 384-well plates (~ 10 min/plate). Used for both cell- and bead-based HTS. |
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Plate reader: PHERAstar (BMG Labs) with luminescence, fluorescence, fluorescence polarization, time-resolved fluorescence and absorbance detection methods with 96 and 384-well plate capability. Sample sizes from 5-200ul. |
Pherastar Modules:
Other filter modules available request a list from Martha Larsen.
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FlexStation3 (MD) plate reader that is capable of reading 5 assay formats for kinetic, endpoint, spectrum, well-scanning and “Flex.”. |
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Plate Handler: Twister (Caliper Life Sciences) microplate handler allows for automated integration of liquid dispensers, plate washers, and other HTS assay components during screening creating a stand alone module. |
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Bio-Tek ELx405 plate washer/aspirator with 96 to 384-well capability. |
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Thermo Labsystems Multidrop plate dispensers with 96 and 384-well capability. |
Tissue culture facilities. Multichannel pipettors.
Updated August, 2011.
